The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.
The heterodimeric transcription elongation factor Spt4/Spt5 (Spt4/5) tightly associates with RNAPII to regulate both transcriptional elongation and co-transcriptional pre-mRNA processing; however, the mechanisms by which Spt4/5 acts are poorly understood. Recent studies of the human and Drosophila Spt4/5 complexes indicate that they can bind nucleic acids in vitro. We demonstrate ... here that yeast Spt4/5 can bind in a sequence-specific manner to single stranded RNA containing AAN repeats. Furthermore, we show that the major protein determinants for RNA-binding are Spt4 together with the NGN domain of Spt5 and that the KOW domains are not required for RNA recognition. These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5.
Mesh Terms:
Animals, Chromosomal Proteins, Non-Histone, Drosophila melanogaster, Humans, Nuclear Proteins, Protein Domains, RNA Polymerase II, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcriptional Elongation Factors
Animals, Chromosomal Proteins, Non-Histone, Drosophila melanogaster, Humans, Nuclear Proteins, Protein Domains, RNA Polymerase II, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcriptional Elongation Factors
Protein Sci.
Date: Sep. 01, 2016
PubMed ID: 27376968
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