The Histone Chaperones FACT and Spt6 Restrict H2A.Z from Intragenic Locations.
H2A.Z is a highly conserved histone variant involved in several key nuclear processes. It is incorporated into promoters by SWR-C-related chromatin remodeling complexes, but whether it is also actively excluded from non-promoter regions is not clear. Here we provide genomic and biochemical evidence that the RNA polymerase II (RNA Pol ... II) elongation-associated histone chaperones FACT and Spt6 both contribute to restricting H2A.Z from intragenic regions. In the absence of FACT or Spt6, the lack of efficient nucleosome reassembly coupled to pervasive incorporation of H2A.Z by mislocalized SWR-C alters chromatin composition and contributes to cryptic initiation. Therefore, chaperone-mediated H2A.Z confinement is crucial for restricting the chromatin signature of gene promoters that otherwise may license or promote cryptic transcription.
Mesh Terms:
Blotting, Western, Chromatin, Gene Expression Regulation, Fungal, Histone Chaperones, Histones, Models, Genetic, Mutation, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, RNA Polymerase II, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic, Transcriptional Elongation Factors
Blotting, Western, Chromatin, Gene Expression Regulation, Fungal, Histone Chaperones, Histones, Models, Genetic, Mutation, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, RNA Polymerase II, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic, Transcriptional Elongation Factors
Mol. Cell
Date: Jun. 18, 2015
PubMed ID: 25959393
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