A generic protein purification method for protein complex characterization and proteome exploration.

We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or ...
function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
Mesh Terms:
Affinity Labels, Amino Acid Sequence, Electrophoresis, Polyacrylamide Gel, Methods, Molecular Sequence Data, Proteins, Proteome
Nat. Biotechnol.
Date: Oct. 01, 1999
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