Perturbation of protein kinase CK2 uncouples executive part of phosphate maintenance pathway from cyclin-CDK control.
The budding yeast Saccharomyces cerevisiae encounters phosphate starvation by the transcription-regulated PHO pathway. We find that genetic perturbation of protein kinase CK2, a conserved tetrameric Ser/Thr phosphotransferase with links to cell cycle and transcription, affects expression of PHO pathway genes in a subunit- and isoform-specific manner. Remarkably, the genes encoding ... phosphate supplying phosphatases and transporters are significantly repressed, while the genes encoding components of the central pathway regulator complex, a cyclin-dependent kinase (CDK), a cyclin, and a CDK inhibitor, remain unaltered. Thus, perturbation of CK2 uncouples the executive part of the PHO pathway from its cyclin-CDK control complex.
Mesh Terms:
Base Sequence, Casein Kinase II, Cell Cycle, Cyclin-Dependent Kinases, Cyclins, DNA Primers, Models, Biological, Open Reading Frames, Phosphates, Protein-Serine-Threonine Kinases, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae
Base Sequence, Casein Kinase II, Cell Cycle, Cyclin-Dependent Kinases, Cyclins, DNA Primers, Models, Biological, Open Reading Frames, Phosphates, Protein-Serine-Threonine Kinases, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae
FEBS Lett.
Date: Feb. 27, 2003
PubMed ID: 12606059
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