Regulation of the interaction between the neuronal BIN1 isoform 1 and Tau proteins - role of the SH3 domain.
Bridging integrator 1 (bin1) gene is a genetic determinant of Alzheimer's disease (AD) and has been reported to modulate Alzheimer's pathogenesis through pathway(s) involving Tau. The functional impact of Tau/BIN1 interaction as well as the molecular details of this interaction are still not fully resolved. As a consequence, how BIN1 ... through its interaction with Tau affects AD risk is also still not determined. To progress in this understanding, interaction of Tau with two BIN1 isoforms was investigated using Nuclear Magnetic Resonance spectroscopy. 1 H, 15 N spectra showed that the C-terminal SH3 domain of BIN1 isoform 1 (BIN1Iso1) is not mobile in solution but locked with the core of the protein. In contrast, the SH3 domain of BIN1 isoform 9 (BIN1Iso9) behaves as an independent mobile domain. This reveals an equilibrium between close and open conformations for the SH3 domain. Interestingly, a 334-376 peptide from the clathrin and AP-2-binding domain (CLAP) domain of BIN1Iso1, which contains a SH3-binding site, is able to compete with BIN1-SH3 intramolecular interaction. For both BIN1 isoforms, the SH3 domain can interact with Tau(210-240) sequence. Tau(210-240) peptide can indeed displace the intramolecular interaction of the BIN1-SH3 of BIN1Iso1 and form a complex with the released domain. The measured Kd were in agreement with a stronger affinity of Tau peptide. Both CLAP and Tau peptides occupied the same surface on the BIN1-SH3 domain, showing that their interaction is mutually exclusive. These results emphasize an additional level of complexity in the regulation of the interaction between BIN1 and Tau dependent of the BIN1 isoforms.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Binding Sites, Cloning, Molecular, Escherichia coli, Gene Expression, Humans, Kinetics, Models, Molecular, Neurons, Nuclear Magnetic Resonance, Biomolecular, Nuclear Proteins, Peptides, Protein Binding, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Protein Isoforms, Recombinant Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Tumor Suppressor Proteins, tau Proteins
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Binding Sites, Cloning, Molecular, Escherichia coli, Gene Expression, Humans, Kinetics, Models, Molecular, Neurons, Nuclear Magnetic Resonance, Biomolecular, Nuclear Proteins, Peptides, Protein Binding, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Protein Isoforms, Recombinant Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Tumor Suppressor Proteins, tau Proteins
FEBS J.
Date: Dec. 01, 2016
PubMed ID: 28755476
View in: Pubmed Google Scholar
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