ATP-independent control of Vac8 palmitoylation by a SNARE subcomplex on yeast vacuoles.

Yeast vacuole fusion requires palmitoylated Vac8. We previously showed that Vac8 acylation occurs early in the fusion reaction, is blocked by antibodies against Sec18 (yeast N-ethylmaleimide-sensitive fusion protein (NSF)), and is mediated by the R-SNARE Ykt6. Here we analyzed the regulation of this reaction on purified vacuoles. We show that ...
Vac8 acylation is restricted to a narrow time window, is independent of ATP hydrolysis by Sec18, and is stimulated by the ion chelator EDTA. Analysis of vacuole protein complexes indicated that Ykt6 is part of a complex distinct from the second R-SNARE, Nyv1. We speculate that during vacuole fusion, Nyv1 is the classical R-SNARE, whereas the Ykt6-containing complex has a novel function in Vac8 palmitoylation.
Mesh Terms:
Adenosine Triphosphatases, Adenosine Triphosphate, Dose-Response Relationship, Drug, Edetic Acid, Electrophoresis, Polyacrylamide Gel, Genotype, Glutathione Transferase, Hydrolysis, Immunoprecipitation, Lipoproteins, Membrane Proteins, Microscopy, Phase-Contrast, Models, Biological, Palmitic Acid, Protein Binding, R-SNARE Proteins, Recombinant Proteins, SNARE Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors, Vacuoles, Vesicular Transport Proteins
J. Biol. Chem.
Date: Apr. 15, 2005
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