Functional link between ribosome formation and biogenesis of iron-sulfur proteins.
In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron-sulfur (Fe-S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe-S clusters within Rli1, a potential substrate protein of ... unknown function. We tested whether the Fe-S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in pre-rRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe-S proteins.
Mesh Terms:
ATP-Binding Cassette Transporters, Adenosine Triphosphatases, Base Sequence, Biological Transport, Active, DNA, Fungal, Eukaryotic Initiation Factor-3, GTP-Binding Proteins, Genes, Fungal, Iron-Sulfur Proteins, Mutation, RNA Precursors, RNA, Fungal, RNA, Ribosomal, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
ATP-Binding Cassette Transporters, Adenosine Triphosphatases, Base Sequence, Biological Transport, Active, DNA, Fungal, Eukaryotic Initiation Factor-3, GTP-Binding Proteins, Genes, Fungal, Iron-Sulfur Proteins, Mutation, RNA Precursors, RNA, Fungal, RNA, Ribosomal, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
EMBO J.
Date: Feb. 09, 2005
PubMed ID: 15660135
View in: Pubmed Google Scholar
Download Curated Data For This Publication
20912
Switch View:
- Interactions 2