NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling.
NDRG2 is a member of the N-myc downstream regulated gene (NDRG) family, implicated in cell growth and differentiation. Investigation of NDRG2 molecular interactions by yeast two-hybrid screening identified prenylated Rab acceptor-1 (PRA1), involved in vesicle trafficking and protein transport, as binding partner. Binding of NDRG2 (and NDRG1-4) with PRA1 in ... vitro was confirmed by GST pull-down assay and immunoprecipitation, and colocalization was verified by confocal microscopy in HCT116 cells. Intracellular coexpression showed that NDRG2 and PRA1 synergistically downregulate T-cell factor (TCF) promoter activity and GSK3β phosphorylation. Results suggest that NDRG2 and PRA1 might act synergistically to prevent signaling of TCF/β-catenin.
Mesh Terms:
Active Transport, Cell Nucleus, Binding Sites, Blotting, Western, Cell Nucleus, Colorectal Neoplasms, GTP-Binding Proteins, Gene Expression Regulation, Glycogen Synthase Kinase 3, Glycogen Synthase Kinase 3 beta, Green Fluorescent Proteins, HCT116 Cells, Humans, Microscopy, Confocal, Mutation, Phosphorylation, Promoter Regions, Genetic, Protein Binding, Signal Transduction, TCF Transcription Factors, Tumor Suppressor Proteins, Two-Hybrid System Techniques, Vesicular Transport Proteins, beta Catenin
Active Transport, Cell Nucleus, Binding Sites, Blotting, Western, Cell Nucleus, Colorectal Neoplasms, GTP-Binding Proteins, Gene Expression Regulation, Glycogen Synthase Kinase 3, Glycogen Synthase Kinase 3 beta, Green Fluorescent Proteins, HCT116 Cells, Humans, Microscopy, Confocal, Mutation, Phosphorylation, Promoter Regions, Genetic, Protein Binding, Signal Transduction, TCF Transcription Factors, Tumor Suppressor Proteins, Two-Hybrid System Techniques, Vesicular Transport Proteins, beta Catenin
FEBS Lett.
Date: Nov. 16, 2012
PubMed ID: 23068607
View in: Pubmed Google Scholar
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