Involvement of DNA mismatch repair in the maintenance of heterochromatic DNA stability in Saccharomyces cerevisiae.
Heterochromatin contains a significant part of nuclear DNA. Little is known about the mechanisms that govern heterochromatic DNA stability. We show here that in the yeast Saccharomyces cerevisiae (i) DNA mismatch repair (MMR) is required for the maintenance of heterochromatic DNA stability, (ii) MutLα (Mlh1-Pms1 heterodimer), MutSα (Msh2-Msh6 heterodimer), MutSβ ... (Msh2-Msh3 heterodimer), and Exo1 are involved in MMR at heterochromatin, (iii) Exo1-independent MMR at heterochromatin frequently leads to the formation of Pol ζ-dependent mutations, (iv) MMR cooperates with the proofreading activity of Pol ε and the histone acetyltransferase Rtt109 in the maintenance of heterochromatic DNA stability, (v) repair of base-base mismatches at heterochromatin is less efficient than repair of base-base mismatches at euchromatin, and (vi) the efficiency of repair of 1-nt insertion/deletion loops at heterochromatin is similar to the efficiency of repair of 1-nt insertion/deletion loops at euchromatin.
Mesh Terms:
Base Sequence, DNA Damage, DNA Mismatch Repair, DNA, Fungal, Exodeoxyribonucleases, Genes, pol, Heterochromatin, Histone Acetyltransferases, MutL Proteins, MutS DNA Mismatch-Binding Protein, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology
Base Sequence, DNA Damage, DNA Mismatch Repair, DNA, Fungal, Exodeoxyribonucleases, Genes, pol, Heterochromatin, Histone Acetyltransferases, MutL Proteins, MutS DNA Mismatch-Binding Protein, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology
PLoS Genet.
Date: Oct. 01, 2017
PubMed ID: 29069084
View in: Pubmed Google Scholar
Download Curated Data For This Publication
209618
Switch View:
- Interactions 4