Membrane protein insertion through a mitochondrial β-barrel gate.
The biogenesis of mitochondria, chloroplasts, and Gram-negative bacteria requires the insertion of β-barrel proteins into the outer membranes. Homologous Omp85 proteins are essential for membrane insertion of β-barrel precursors. It is unknown if precursors are threaded through the Omp85-channel interior and exit laterally or if they are translocated into the ... membrane at the Omp85-lipid interface. We have mapped the interaction of a precursor in transit with the mitochondrial Omp85-channel Sam50 in the native membrane environment. The precursor is translocated into the channel interior, interacts with an internal loop, and inserts into the lateral gate by β-signal exchange. Transport through the Omp85-channel interior followed by release through the lateral gate into the lipid phase may represent a basic mechanism for membrane insertion of β-barrel proteins.
Mesh Terms:
Mitochondria, Mitochondrial Membrane Transport Proteins, Mitochondrial Membranes, Mitochondrial Proteins, Porins, Protein Conformation, beta-Strand, Protein Folding, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Voltage-Dependent Anion Channel 1
Mitochondria, Mitochondrial Membrane Transport Proteins, Mitochondrial Membranes, Mitochondrial Proteins, Porins, Protein Conformation, beta-Strand, Protein Folding, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Voltage-Dependent Anion Channel 1
Science
Date: Dec. 19, 2017
PubMed ID: 29348211
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