Cobalt chloride-mediated protein kinase Cα (PKCα) phosphorylation induces hypoxia-inducible factor 1α (HIF1α) in the nucleus of gastric cancer cell.

Hypoxia promotes cancer progression, and metastasis. The major protein expressed in hypoxic solid cancer is hypoxia-inducible factor 1 (HIF1). We show that enhanced phosphorylation of a conventional protein kinase C isoform, PKCα, at threonine 638 (T(638)) by hypoxia-mimetic cobalt chloride induces HIF1α in nuclei of gastric epithelial cells (GECs). Moreover, ...
phospho-T(638)-PKCα (P-PKCα) interacts with p300-HIF1α complex in the nuclei of hypoxic GECs and PKCα phosphorylation at T(638) enhances transcriptional activity of HIF1α. High P-PKCα expression in neoplastic gastric cancer biopsy samples as compared to nonneoplastic samples suggests that P-PKCα might act as an indicator of gastric cancer progression.
Mesh Terms:
Cell Hypoxia, Cell Line, Cell Nucleus, Cells, Cultured, Cobalt, Dose-Response Relationship, Drug, Gastric Mucosa, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Phosphorylation, Protein Kinase C-alpha
Biochem. Biophys. Res. Commun.
Date: Feb. 26, 2016
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