C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.

The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex siRNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used ...
this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.
Mesh Terms:
Amino Acid Sequence, Animals, Argonaute Proteins, Carrier Proteins, Catalytic Domain, Drosophila Proteins, Drosophila melanogaster, Models, Molecular, Molecular Sequence Data, Mutation, Protein Conformation, RNA Helicases, RNA Interference, RNA, Double-Stranded, RNA, Small Interfering, RNA-Binding Proteins, RNA-Induced Silencing Complex, Recombinant Proteins, Ribonuclease III
Science
Date: Aug. 07, 2009
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