The protein kinase Pelle mediates feedback regulation in the Drosophila Toll signaling pathway.

Dorsoventral polarity in the Drosophila embryo is established through a signal transduction cascade triggered in ventral and ventrolateral regions. Activation of a transmembrane receptor, Toll, leads to localized recruitment of the adaptor protein Tube and protein kinase Pelle. Signaling through these components directs degradation of the IkappaB-like inhibitor Cactus and ...
nuclear translocation of the Rel protein Dorsal. Here we show through confocal immunofluorescence microscopy that Pelle functions to downregulate the signal-dependent relocalization of Tube. Inactivation of the Pelle kinase domain, or elimination of the Tube-Pelle interaction, dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling. We also characterize a large collection of pelle alleles, identifying the molecular lesions in these alleles and their effects on Pelle autophosphorylation, Tube phosphorylation and Tube relocalization. Our results point to a mechanism operating to modulate the domain or duration of signaling downstream from Tube and Pelle.
Mesh Terms:
Alleles, Amino Acid Sequence, Animals, Body Patterning, Drosophila Proteins, Drosophila melanogaster, Feedback, Genes, Insect, Insect Proteins, Mammals, Membrane Glycoproteins, Models, Biological, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Protein-Serine-Threonine Kinases, Receptors, Cell Surface, Sequence Homology, Amino Acid, Signal Transduction, Toll-Like Receptors
Development
Date: Dec. 01, 2001
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