Bicaudal-C associates with a Trailer Hitch/Me31B complex and is required for efficient Gurken secretion.

Bicaudal-C (Bic-C) is a multiple KH-domain RNA-binding protein required for Drosophila oogenesis and, maternally, for embryonic patterning. In early oogenesis, Bic-C negatively regulates target mRNAs, including Bic-C, by recruiting the CCR4 deadenylase through a direct association with its NOT3 subunit. Here, we identify a novel function for Bic-C in secretion ...
of the TGF-alpha homolog Gurken (Grk). In Bic-C mutant egg chambers, Grk is sequestered within actin-coated structures during mid-oogenesis. As a consequence, Egfr signalling is not efficiently activated in the dorsal-anterior follicle cells. This phenotype is strikingly similar to that of trailer hitch (tral) mutants. Consistent with the idea that Bic-C and Tral act together in Grk secretion, Bic-C co-localizes with Tral within cytoplasmic granules, and can be co-purified with multiple protein components of a Tral mRNP complex. Taken together, our results implicate translational regulation by Bic-C and Tral in the secretory pathway.
Mesh Terms:
Animals, DEAD-box RNA Helicases, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Mutation, Oogenesis, RNA-Binding Proteins, Ribonucleoproteins, Transforming Growth Factor alpha
Dev. Biol.
Date: Apr. 01, 2009
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