Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor.
We provide evidence that Prp4p kinase activity is required for pre-mRNA splicing in vivo and show that loss of activity impairs G1-S and G2-M progression in the cell cycle. Prp4p interacts genetically with the non-SR (serine/arginine) splicing factors Prp1p and Prp5p. Bacterially produced Prp1p is phosphorylated by Prp4p in vitro. ... Prp4p and Prp1p also interact in the yeast two-hybrid system. In vivo labelling studies using a strain with a mutant allele of the prp4 gene in the genetic background indicate a change in phosphorylation of the Prp1p protein. These results are consistent with the notion that Prp4p kinase is involved in the control of the formation of active spliceosomes, targeting non-SR splicing factors.
Mesh Terms:
Alleles, DEAD-box RNA Helicases, Fungal Proteins, G1 Phase, G2 Phase, Mutagenesis, Mutation, Phenotype, Phosphorylation, Plasmids, Protein Binding, Protein-Serine-Threonine Kinases, RNA Helicases, RNA Splicing, RNA, Messenger, Ribonucleoprotein, U4-U6 Small Nuclear, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Spliceosomes, Temperature, Time Factors, Two-Hybrid System Techniques
Alleles, DEAD-box RNA Helicases, Fungal Proteins, G1 Phase, G2 Phase, Mutagenesis, Mutation, Phenotype, Phosphorylation, Plasmids, Protein Binding, Protein-Serine-Threonine Kinases, RNA Helicases, RNA Splicing, RNA, Messenger, Ribonucleoprotein, U4-U6 Small Nuclear, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Spliceosomes, Temperature, Time Factors, Two-Hybrid System Techniques
EMBO Rep.
Date: Jan. 01, 2001
PubMed ID: 11252721
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