Transcriptional termination factors for RNA polymerase II in yeast.
The molecular connections between mRNA 3' end processing and transcriptional termination have been investigated in S. pombe using a genetic screen. By this approach, we have identified a RNAP II termination domain in the well-defined cleavage polyadenylation factor called CstF-64 in metazoans and Rna15p in S. cerevisiae. Furthermore, this C-terminal ... domain interacts with Res2, previously identified as a component of the G1/S transition-specific transcription factor MBF. Deletion of res2 in both fission and budding yeast results in a defect in 3' end formation. This raises the possibility that RNAP II transcriptional termination may in some situations be integrated with cell cycle events.
Mesh Terms:
3' Untranslated Regions, Amino Acid Sequence, Cell Cycle, DNA-Binding Proteins, Fungal Proteins, Gene Deletion, Molecular Sequence Data, Peptide Termination Factors, RNA Polymerase II, RNA-Binding Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Terminator Regions, Genetic, Transcription Factors, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
3' Untranslated Regions, Amino Acid Sequence, Cell Cycle, DNA-Binding Proteins, Fungal Proteins, Gene Deletion, Molecular Sequence Data, Peptide Termination Factors, RNA Polymerase II, RNA-Binding Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Terminator Regions, Genetic, Transcription Factors, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Mol. Cell
Date: May. 01, 2001
PubMed ID: 11389847
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