Smurf1 controls S phase progression and tumorigenesis through Wee1 degradation.
Smad ubiquitination regulatory factor 1 (Smurf1) is a HECT-type E3 ubiquitin ligase that regulates several important signaling pathways, including the bone morphogenetic protein pathway and the transforming growth factor-beta (TGF-β) signaling pathway. However, the function of Smurf1 in cell cycle progression remains unclear. Here, we demonstrate that silencing of Smurf1 ... results in S phase arrest, confirming that Smurf1 is required for S phase progression. Furthermore, we demonstrate that Smurf1-mediated S phase progression is largely dependent on the ubiquitination-dependent degradation of Wee1. This study defines a novel role for Smurf1 in controlling S phase progression by promoting Wee1 degradation.
Mesh Terms:
A549 Cells, Animals, Carcinogenesis, Cell Cycle Proteins, Cell Proliferation, Enzyme Stability, HEK293 Cells, Half-Life, HeLa Cells, Humans, Immunoprecipitation, Mice, Nude, Neoplasm Transplantation, Nuclear Proteins, Protein-Tyrosine Kinases, Proteolysis, RNA Interference, Recombinant Fusion Proteins, S Phase, Tumor Burden, Ubiquitin-Protein Ligases, Ubiquitination
A549 Cells, Animals, Carcinogenesis, Cell Cycle Proteins, Cell Proliferation, Enzyme Stability, HEK293 Cells, Half-Life, HeLa Cells, Humans, Immunoprecipitation, Mice, Nude, Neoplasm Transplantation, Nuclear Proteins, Protein-Tyrosine Kinases, Proteolysis, RNA Interference, Recombinant Fusion Proteins, S Phase, Tumor Burden, Ubiquitin-Protein Ligases, Ubiquitination
FEBS Lett.
Date: Dec. 01, 2016
PubMed ID: 28294307
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