Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target.
S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal ... region (residues 1-30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, A11, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1-30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function.
Mesh Terms:
Animals, COS Cells, Cell Cycle Proteins, Cells, Cultured, Centrosome, Cercopithecus aethiops, HeLa Cells, Humans, Protein Array Analysis, Protein Binding, Proteins, S100 Calcium Binding Protein A6, S100 Proteins, Substrate Specificity
Animals, COS Cells, Cell Cycle Proteins, Cells, Cultured, Centrosome, Cercopithecus aethiops, HeLa Cells, Humans, Protein Array Analysis, Protein Binding, Proteins, S100 Calcium Binding Protein A6, S100 Proteins, Substrate Specificity
Biochem. Biophys. Res. Commun.
Date: Dec. 30, 2016
PubMed ID: 28765046
View in: Pubmed Google Scholar
Download Curated Data For This Publication
211828
Switch View:
- Interactions 8