Parkin targets HIF-1α for ubiquitination and degradation to inhibit breast tumor progression.

Mutations in E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Accumulating evidence suggests that Parkin is a tumor suppressor, but the underlying mechanism is poorly understood. Here we show that Parkin is an E3 ubiquitin ligase for hypoxia-inducible factor 1α (HIF-1α). Parkin interacts with HIF-1α and promotes ...
HIF-1α degradation through ubiquitination, which in turn inhibits metastasis of breast cancer cells. Parkin downregulation in breast cancer cells promotes metastasis, which can be inhibited by targeting HIF-1α with RNA interference or the small-molecule inhibitor YC-1. We further identify lysine 477 (K477) of HIF-1α as a major ubiquitination site for Parkin. K477R HIF-1α mutation and specific cancer-associated Parkin mutations largely abolish the functions of Parkin to ubiquitinate HIF-1α and inhibit cancer metastasis. Importantly, Parkin expression is inversely correlated with HIF-1α expression and metastasis in breast cancer. Our results reveal an important mechanism for Parkin in tumor suppression and HIF-1α regulation.
Mesh Terms:
Animals, Breast Neoplasms, Cell Line, Tumor, Cell Movement, Down-Regulation, Female, Heterografts, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Indazoles, Lung Neoplasms, MCF-7 Cells, Mice, Mice, Inbred BALB C, Mutation, Neoplasm Metastasis, Transfection, Ubiquitin-Protein Ligases, Ubiquitination
Nat Commun
Date: Dec. 28, 2016
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