Method for the Purification of Endogenous Unanchored Polyubiquitin Chains.
Unanchored polyubiquitin chains are endogenous non-substrate linked ubiquitin polymers which have emerging roles in the control of cellular physiology. We describe an affinity purification method based on an isolated ubiquitin-binding domain, the ZnF_UBP domain of the deubiquitinating enzyme USP5, which permits the selective purification of mixtures of endogenous unanchored polyubiquitin ... chains that are amenable to downstream molecular analyses. Further, we present methods for detection of unanchored polyubiquitin chains in purified fractions.
Mesh Terms:
Animals, Humans, Polyubiquitin, Protein Binding, Proteomics, Ubiquitin, Ubiquitin-Specific Proteases, Ubiquitinated Proteins, Ubiquitination
Animals, Humans, Polyubiquitin, Protein Binding, Proteomics, Ubiquitin, Ubiquitin-Specific Proteases, Ubiquitinated Proteins, Ubiquitination
Methods Mol. Biol.
Date: Sep. 11, 2016
PubMed ID: 27613037
View in: Pubmed Google Scholar
Download Curated Data For This Publication
212055
Switch View:
- Interactions 2