The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.
We have cloned and characterized the fission yeast cdc19+ gene. We demonstrate that it encodes a structural homologue of the budding yeast MCM2 protein. In fission yeast, the cdc19+ gene is constitutively expressed, and essential for viability. Deletion delays progression through S phase, and cells arrest in the first cycle ... with an apparent 2C DNA content, with their checkpoint control intact. The temperature-sensitive cdc19-P1 mutation is synthetically lethal with cdc21-M68. In addition, we show by classical and molecular genetics that cdc19+ is allelic to the nda1+ locus. We conclude that cdc19p plays a potentially conserved role in S phase.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Replication, Fungal Proteins, G1 Phase, Gene Deletion, Genes, Fungal, Genetic Code, Molecular Sequence Data, Multigene Family, Phenotype, S Phase, Schizosaccharomyces, Sequence Homology, Amino Acid
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Replication, Fungal Proteins, G1 Phase, Gene Deletion, Genes, Fungal, Genetic Code, Molecular Sequence Data, Multigene Family, Phenotype, S Phase, Schizosaccharomyces, Sequence Homology, Amino Acid
J. Cell. Sci.
Date: Oct. 01, 1994
PubMed ID: 7876346
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