The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control.
The fission yeast mutant dis3-54 is defective in mitosis and fails in chromosome disjunction. Its phenotype is similar to that of dis2-11, a mutant with a mutation in the type 1 protein phosphatase gene. We cloned the dis3+ gene by transformation. Nucleotide sequencing predicts a coding region of 970 amino ... acids interrupted by a 164-bp intron at the 65th codon. The predicted dis3+ protein shares a weak but significant similarity with the budding yeast SSD1 or SRK1 gene product, the gene for which is a suppressor for the absence of a protein phosphatase SIT4 gene or the BCY1 regulatory subunit of cyclic AMP-dependent protein kinase. Anti-dis3 antibodies recognized the 110-kDa dis3+ gene product, which is part of a 250- to 350-kDa oligomer and is enriched in the nucleus. The cellular localization of the dis3+ protein is reminiscent of that of the dis2+ protein, but these two proteins do not form a complex. A type 1 protein phosphatase activity in the dis3-54 mutant extracts is apparently not affected. The dis3+ gene is essential for growth; gene disruptant cells do not germinate and fail in cell division. Increased dis3+ gene dosage reverses the Ts+ phenotype of a cdc25 wee1 strain, as does increased type 1 protein phosphatase gene dosage. Double mutant dis3 dis2 is lethal even at the permissive temperature, suggesting that the dis2+ and dis3+ genes may be functionally overlapped. The role of the dis3+ gene product in mitosis is unknown, but this gene product may be directly or indirectly involved in the regulation of mitosis.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Cell Nucleus, Chromatography, Gel, Cloning, Molecular, DNA, Fungal, Exoribonucleases, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Restriction Mapping, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment
Amino Acid Sequence, Base Sequence, Cell Nucleus, Chromatography, Gel, Cloning, Molecular, DNA, Fungal, Exoribonucleases, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Restriction Mapping, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment
Mol. Cell. Biol.
Date: Dec. 01, 1991
PubMed ID: 1944266
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