A conserved small GTP-binding protein Alp41 is essential for the cofactor-dependent biogenesis of microtubules in fission yeast.
The proper folding of tubulins and their incorporation into microtubules consist of a series of reactions, in which evolutionarily conserved proteins, cofactors A to E, play a vital role. We have cloned a fission yeast gene (alp41(+)) which encodes a highly conserved small GTP-binding protein homologous to budding yeast CIN4 ... and human ARF-like Arl2. alp41(+) is essential, disruption of which results in microtubule dysfunction and growth polarity defects. Genetic analysis indicates that Alp41 plays a crucial role in the cofactor-dependent pathway, in which it functions upstream of the cofactor D homologue Alp1(D) and possibly in concert with Alp21(E).
Mesh Terms:
Cell Division, Cloning, Molecular, Conserved Sequence, Fungal Proteins, GTP-Binding Proteins, Gene Deletion, Humans, Microtubule-Associated Proteins, Microtubules, Molecular Sequence Data, Phylogeny, Protein Folding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Sequence Homology, Amino Acid
Cell Division, Cloning, Molecular, Conserved Sequence, Fungal Proteins, GTP-Binding Proteins, Gene Deletion, Humans, Microtubule-Associated Proteins, Microtubules, Molecular Sequence Data, Phylogeny, Protein Folding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment, Sequence Homology, Amino Acid
FEBS Lett.
Date: Feb. 18, 2000
PubMed ID: 10683446
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