The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.

We describe a novel set of oscillation mechanisms for the fission yeast S phase cyclin Cig2, which contains an authentic destruction box and is destroyed at anaphase via the APC/cyclosome (APC/C). Unlike the mitotic cyclin Cdc13, however, Cig2 mRNA and protein peak at the G1/S boundary and decline to low ...
levels in G2 and M phases. We show here that SCF(Pop1, Pop2) plays a role in transcriptional periodicity, as pop mutations result in constitutive cig2(+) transcripts. The instability of Cig2 during G2 and M is independent of either the APC/C or Pop1/Pop2, but requires Skp1, a core component of SCF. These data indicate that the APC/C and SCF control Cig2 levels differentially at different stages of the cell cycle.
Mesh Terms:
Anaphase, Animals, CDC2 Protein Kinase, Cell Cycle Proteins, Cyclin B, Cyclins, Cysteine Endopeptidases, Fluorescent Antibody Technique, Fungal Proteins, G1 Phase, G2 Phase, Gene Expression Regulation, Fungal, Half-Life, Ligases, Mitosis, Multienzyme Complexes, Mutation, Peptide Synthases, Proteasome Endopeptidase Complex, Protein Binding, RNA, Messenger, Ribonucleases, Ribonucleoproteins, S Phase, SKP Cullin F-Box Protein Ligases, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transcription Factors, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Xenopus
Mol. Cell
Date: Dec. 01, 2000
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