A centrosomal protein FOR20 regulates microtubule assembly dynamics and plays a role in cell migration.

Here, we report that a centrosomal protein FOR20 [FOP (FGFR1 (fibroblast growth factor receptor 1) oncogene protein)-like protein of molecular mass of 20 kDa; also named as C16orf63, FLJ31153 or PHSECRG2] can regulate the assembly and stability of microtubules. Both FOR20 IgG antibody and GST (glutathione S-transferase)-tagged FOR20 could precipitate tubulin ...
from the HeLa cell extract, indicating a possible interaction between FOR20 and tubulin. FOR20 was also detected in goat brain tissue extract and it cycled with microtubule-associated proteins. Furthermore, FOR20 bound to purified tubulin and inhibited the assembly of tubulin in vitro. The overexpression of FOR20 depolymerized interphase microtubules and the depletion of FOR20 prevented nocodazole-induced depolymerization of microtubules in HeLa cells. In addition, the depletion of FOR20 suppressed the dynamics of individual microtubules in live HeLa cells. FOR20-depleted MDA-MB-231 cells displayed zigzag motion and migrated at a slower rate than the control cells, indicating that FOR20 plays a role in directed cell migration. The results suggested that the centrosomal protein FOR20 is a new member of the microtubule-associated protein family and that it regulates the assembly and dynamics of microtubules.
Mesh Terms:
Animals, Brain Chemistry, Cell Line, Tumor, Cell Movement, Cilia, Epithelial Cells, Focal Adhesions, Gene Expression, Glutathione Transferase, Goats, Green Fluorescent Proteins, HeLa Cells, Humans, Mice, Microtubule-Associated Proteins, Microtubules, NIH 3T3 Cells, Nocodazole, Nuclear Proteins, Proteins, Recombinant Fusion Proteins, Time-Lapse Imaging, Transcription Factors, Tubulin
Biochem. J.
Date: Dec. 10, 2016
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