Myc promotes glutaminolysis in human neuroblastoma through direct activation of glutaminase 2.

Deamidation of glutamine to glutamate by glutaminase 1 (GLS1, also called GLS) and GLS2 is an essential step in both glutaminolysis and glutathione (GSH) biosynthesis. However, mechanisms whereby cancer cells regulate glutamine catabolism remains largely unknown. We report here that N-Myc, an essential Myc family member, promotes conversion of glutamine ...
to glutamate in MYCN-amplified neuroblastoma cells by directly activating GLS2, but not GLS1, transcription. Abrogation of GLS2 function profoundly inhibited glutaminolysis, which resulted in feedback inhibition of aerobic glycolysis likely due to thioredoxin-interacting protein (TXNIP) activation, dramatically decreasing cell proliferation and survival in vitro and in vivo. Moreover, elevated GLS2 expression is significantly elevated in MYCN-amplified neuroblastomas in comparison with non-amplified ones, correlating with unfavorable patient survival. In aggregate, these results reveal a novel mechanism deciphering context-dependent regulation of metabolic heterogeneities, uncovering a previously unsuspected link between Myc, GLS2 and tumor metabolism.
Mesh Terms:
Animals, Apoptosis, Blotting, Western, Cell Proliferation, Chromatin Immunoprecipitation, Enzyme Activation, Glutaminase, Glutamine, Glycolysis, Humans, Hydrolysis, Immunoenzyme Techniques, Mice, Mice, Inbred NOD, Mice, SCID, Neuroblastoma, Proto-Oncogene Proteins c-myc, RNA, Messenger, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Xenograft Model Antitumor Assays
Oncotarget
Date: Dec. 01, 2015
Download Curated Data For This Publication
213499
Switch View:
  • Interactions 2