Citron Kinase Deficiency Leads to Chromosomal Instability and TP53-Sensitive Microcephaly.

Mutations in citron (CIT), leading to loss or inactivation of the citron kinase protein (CITK), cause primary microcephaly in humans and rodents, associated with cytokinesis failure and apoptosis in neural progenitors. We show that CITK loss induces DNA damage accumulation and chromosomal instability in both mammals and Drosophila. CITK-deficient cells ...
display "spontaneous" DNA damage, increased sensitivity to ionizing radiation, and defective recovery from radiation-induced DNA lesions. In CITK-deficient cells, DNA double-strand breaks increase independently of cytokinesis failure. Recruitment of RAD51 to DNA damage foci is compromised by CITK loss, and CITK physically interacts with RAD51, suggesting an involvement of CITK in homologous recombination. Consistent with this scenario, in doubly CitK and Trp53 mutant mice, neural progenitor cell death is dramatically reduced; moreover, clinical and neuroanatomical phenotypes are remarkably improved. Our results underscore a crucial role of CIT in the maintenance of genomic integrity during brain development.
Mesh Terms:
Animals, Chromosomal Instability, Cytokinesis, DNA Breaks, Double-Stranded, DNA Damage, DNA Repair, Drosophila, Homologous Recombination, Intracellular Signaling Peptides and Proteins, Mammals, Mice, Microcephaly, Protein-Serine-Threonine Kinases, Rad51 Recombinase, Radiation, Ionizing, Tumor Suppressor Protein p53
Cell Rep
Date: Dec. 14, 2016
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