Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.

MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.
Mesh Terms:
Amino Acid Motifs, Cell Cycle Proteins, Cysteine Endopeptidases, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Genes, Reporter, Multienzyme Complexes, Phenotype, Proteasome Endopeptidase Complex, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Surface Plasmon Resonance, Ubiquitin, Ubiquitins
Nat. Cell Biol. Oct. 01, 2001; 3(10);939-43 [PUBMED:11584278]
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