The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Kinetochores (KTs) are large multiprotein complexes that constitute the interface between centromeric chromatin and the mitotic spindle during chromosome segregation. In spite of their essential role, little is known about how centromeres and KTs are assembled and how their precise stoichiometry is regulated. In this study, we show that the ... nuclear pore basket component Alm1 is required to maintain both the proteasome and its anchor, Cut8, at the nuclear envelope, which in turn regulates proteostasis of certain inner KT components. Consistently, alm1-deleted cells show increased levels of KT proteins, including CENP-CCnp3, spindle assembly checkpoint activation, and chromosome segregation defects. Our data demonstrate a novel function of the nucleoporin Alm1 in proteasome localization required for KT homeostasis.
Mesh Terms:
Carrier Proteins, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, Fungal, Endopeptidases, Genotype, Homeostasis, Kinetochores, Mutation, Nuclear Envelope, Phenotype, Proteasome Endopeptidase Complex, Proteolysis, Schizosaccharomyces, Schizosaccharomyces pombe Proteins
Carrier Proteins, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, Fungal, Endopeptidases, Genotype, Homeostasis, Kinetochores, Mutation, Nuclear Envelope, Phenotype, Proteasome Endopeptidase Complex, Proteolysis, Schizosaccharomyces, Schizosaccharomyces pombe Proteins
J. Cell Biol.
Date: Dec. 06, 2016
PubMed ID: 28974540
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