Sae2 antagonizes Rad9 accumulation at DNA double-strand breaks to attenuate checkpoint signaling and facilitate end resection.

The Mre11-Rad50-Xrs2NBS1 complex plays important roles in the DNA damage response by activating the Tel1ATM kinase and catalyzing 5'-3' resection at DNA double-strand breaks (DSBs). To initiate resection, Mre11 endonuclease nicks the 5' strands at DSB ends in a reaction stimulated by Sae2CtIP Accordingly, Mre11-nuclease deficient (mre11-nd) and sae2Δ mutants ...
are expected to exhibit similar phenotypes; however, we found several notable differences. First, sae2Δ cells exhibit greater sensitivity to genotoxins than mre11-nd cells. Second, sae2Δ is synthetic lethal with sgs1Δ, whereas the mre11-nd sgs1Δ mutant is viable. Third, Sae2 attenuates the Tel1-Rad53CHK2 checkpoint and antagonizes Rad953BP1 accumulation at DSBs independent of Mre11 nuclease. We show that Sae2 competes with other Tel1 substrates, thus reducing Rad9 binding to chromatin and to Rad53. We suggest that persistent Sae2 binding at DSBs in the mre11-nd mutant counteracts the inhibitory effects of Rad9 and Rad53 on Exo1 and Dna2-Sgs1-mediated resection, accounting for the different phenotypes conferred by mre11-nd and sae2Δ mutations. Collectively, these data show a resection initiation independent role for Sae2 at DSBs by modulating the DNA damage checkpoint.
Mesh Terms:
Cell Cycle, Cell Cycle Proteins, Checkpoint Kinase 2, DNA, DNA Breaks, Double-Stranded, DNA Damage, DNA Helicases, DNA Repair, DNA, Fungal, DNA-Binding Proteins, Endodeoxyribonucleases, Endonucleases, Exodeoxyribonucleases, Intracellular Signaling Peptides and Proteins, Mutagens, Protein-Serine-Threonine Kinases, RecQ Helicases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 18, 2017
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