Cyclin D3 interacts with vitamin D receptor and regulates its transcription activity.

D-type cyclins are essential for the progression through the G1 phase of the cell cycle. Besides serving as cell cycle regulators, D-type cyclins were recently reported to have transcription regulation functions. Here, we report that cyclin D3 is a new interacting partner of vitamin D receptor (VDR), a member of ...
the superfamily of nuclear receptors for steroid hormones, thyroid hormone, and the fat-soluble vitamins A and D. The interaction was confirmed with methods of yeast two-hybrid system, in vitro binding analysis and in vivo co-immunoprecipitation. Cyclin D3 interacted with VDR in a ligand-independent manner, but treatment of the ligand, 1,25-dihydroxyvitamin D3, strengthened the interaction. Confocal microscopy analysis showed that ligand-activated VDR led to an accumulation of cyclin D3 in the nuclear region. Cyclin D3 up-regulated transcriptional activity of VDR and this effect was counteracted by overexpression of CDK4 and CDK6. These findings provide us a new clue to understand the transcription regulation functions of D-type cyclins.
Mesh Terms:
Animals, Base Sequence, Cyclin D3, Cyclins, DNA Primers, HeLa Cells, Humans, Immunoprecipitation, Ligands, Mice, NIH 3T3 Cells, Protein Binding, Receptors, Calcitriol, Transcription, Genetic, Two-Hybrid System Techniques, Up-Regulation
Biochem. Biophys. Res. Commun.
Date: Sep. 30, 2005
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