Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.
Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the ... spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.
Mesh Terms:
Anaphase, Calcium-Binding Proteins, Carrier Proteins, Cell Cycle Proteins, Chromosomes, Fungal, DNA Replication, Fungal Proteins, Genes, Fungal, Kinesin, Ligases, Metaphase, Mitosis, Mitotic Spindle Apparatus, Mutagenesis, Site-Directed, Mutation, Nuclear Proteins, Recombinant Fusion Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transformation, Genetic, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases
Anaphase, Calcium-Binding Proteins, Carrier Proteins, Cell Cycle Proteins, Chromosomes, Fungal, DNA Replication, Fungal Proteins, Genes, Fungal, Kinesin, Ligases, Metaphase, Mitosis, Mitotic Spindle Apparatus, Mutagenesis, Site-Directed, Mutation, Nuclear Proteins, Recombinant Fusion Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transformation, Genetic, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases
Science
Date: Feb. 13, 1998
PubMed ID: 9461438
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