Molecular structure of promoter-bound yeast TFIID.
Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better ... than 5 A, except for a flexible domain. We position the crystal structures of several subunits and, in combination with cross-linking studies, describe the quaternary organization of TFIID. The compact tri lobed architecture is stabilized by a topologically closed Taf5-Taf6 tetramer. We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a histone fold domain in the Twin lobe.
Mesh Terms:
DNA, Fungal, Models, Molecular, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Saccharomyces cerevisiae, Transcription Factor TFIID, Yeasts
DNA, Fungal, Models, Molecular, Promoter Regions, Genetic, Protein Binding, Protein Subunits, Saccharomyces cerevisiae, Transcription Factor TFIID, Yeasts
Nat Commun
Date: Dec. 07, 2017
PubMed ID: 30405110
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