A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast.
CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A ... binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Cycle, Centromere, Chromosomal Proteins, Non-Histone, Chromosomes, DNA-Binding Proteins, GATA Transcription Factors, Histones, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Protein Binding, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment
Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Cycle, Centromere, Chromosomal Proteins, Non-Histone, Chromosomes, DNA-Binding Proteins, GATA Transcription Factors, Histones, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Protein Binding, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Alignment
Mol. Cell
Date: Jan. 01, 2003
PubMed ID: 12535531
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