The phosphotyrosine phosphatase SHP-2 participates in a multimeric signaling complex and regulates T cell receptor (TCR) coupling to the Ras/mitogen-activated protein kinase (MAPK) pathway in Jurkat T cells.
Src homology 2 (SH2) domain-containing phosphotyrosine phosphatases (SHPs) are increasingly being shown to play critical roles in protein tyrosine kinase-mediated signaling pathways. The role of SHP-1 as a negative regulator of T cell receptor (TCR) signaling has been established. To further explore the function of the other member of this ... family, SHP-2, in TCR-mediated events, a catalytically inactive mutant SHP-2 was expressed under an inducible promoter in Jurkat T cells. Expression of the mutant phosphatase significantly inhibited TCR-induced activation of the extracellular-regulated kinase (ERK)-2 member of the mitogen-activated protein kinase (MAPK) family, but had no effect on TCR-zeta chain tyrosine phosphorylation or TCR-elicited Ca2+ transients. Inactive SHP-2 was targeted to membranes resulting in the selective increase in tyrosine phosphorylation of three membrane-associated candidate SHP-2 substrates of 110 kD, 55-60 kD, and 36 kD, respectively. Analysis of immunoprecipitates containing inactive SHP-2 also indicated that the 110-kD and 36-kD Grb-2-associated proteins were putative substrates for SHP-2. TCR-stimulation of Jurkat T cells expressing wild-type SHP-2 resulted in the formation of a multimeric cytosolic complex composed of SHP-2, Grb-2, phosphatidylinositol (PI) 3'-kinase, and p110. A significant proportion of this complex was shown to be membrane associated, presumably as a result of translocation from the cytosol. Catalytically inactive SHP-2, rather than the wild-type PTPase, was preferentially localized in complex with Grb-2 and the p85 subunit of PI 3'-kinase, suggesting that the dephosphorylating actions of SHP-2 may regulate the association of these signaling molecules to the p110 complex. Our results show that SHP-2 plays a critical role in linking the TCR to the Ras/MAPK pathway in Jurkat T cells, and also provide some insight into the molecular interactions of SHP-2 that form the basis of this signal transduction process.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Annexin A2, Calcium-Calmodulin-Dependent Protein Kinases, Enzyme Activation, GRB2 Adaptor Protein, Gene Expression Regulation, Neoplastic, Humans, Immunosuppressive Agents, Intracellular Signaling Peptides and Proteins, Jurkat Cells, Mitogen-Activated Protein Kinase 1, Muromonab-CD3, Phosphatidylinositol 3-Kinases, Phosphorylation, Protein Tyrosine Phosphatase, Non-Receptor Type 11, Protein Tyrosine Phosphatase, Non-Receptor Type 6, Protein Tyrosine Phosphatases, Proteins, Receptors, Antigen, T-Cell, SH2 Domain-Containing Protein Tyrosine Phosphatases, Signal Transduction
Adaptor Proteins, Signal Transducing, Annexin A2, Calcium-Calmodulin-Dependent Protein Kinases, Enzyme Activation, GRB2 Adaptor Protein, Gene Expression Regulation, Neoplastic, Humans, Immunosuppressive Agents, Intracellular Signaling Peptides and Proteins, Jurkat Cells, Mitogen-Activated Protein Kinase 1, Muromonab-CD3, Phosphatidylinositol 3-Kinases, Phosphorylation, Protein Tyrosine Phosphatase, Non-Receptor Type 11, Protein Tyrosine Phosphatase, Non-Receptor Type 6, Protein Tyrosine Phosphatases, Proteins, Receptors, Antigen, T-Cell, SH2 Domain-Containing Protein Tyrosine Phosphatases, Signal Transduction
J. Exp. Med.
Date: May. 04, 1998
PubMed ID: 9565634
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