Genome-wide SWAp-Tag yeast libraries for proteome exploration.
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse ... libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
Mesh Terms:
Genetic Complementation Test, Genome, Fungal, Genomic Library, Green Fluorescent Proteins, Mitochondrial Proteins, Nuclear Proteins, Promoter Regions, Genetic, Protein Interaction Mapping, Proteome, Recombinant Fusion Proteins, Ribonucleoproteins, Small Nucleolar, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Tagged Sites
Genetic Complementation Test, Genome, Fungal, Genomic Library, Green Fluorescent Proteins, Mitochondrial Proteins, Nuclear Proteins, Promoter Regions, Genetic, Protein Interaction Mapping, Proteome, Recombinant Fusion Proteins, Ribonucleoproteins, Small Nucleolar, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Tagged Sites
Nat. Methods
Date: Dec. 01, 2017
PubMed ID: 29988094
View in: Pubmed Google Scholar
Download Curated Data For This Publication
216130
Switch View:
- Interactions 41