Roles of Pdk1p, a fission yeast protein related to phosphoinositide-dependent protein kinase, in the regulation of mitosis and cytokinesis.
Proteins related to the phosphoinositide-dependent protein kinase family have been identified in the majority of eukaryotes. Although much is known about upstream mechanisms that regulate the PDK1-family of kinases in metazoans, how these kinases regulate cell growth and division remains unclear. Here, we characterize a fission yeast protein related to ... members of this family, which we have termed Pdk1p. Pdk1p localizes to the spindle pole body and the actomyosin ring in early mitotic cells. Cells deleted for pdk1 display multiple defects in mitosis and cytokinesis, all of which are exacerbated when the function of fission yeast polo kinase, Plo1p, is partially compromised. We conclude that Pdk1p functions in concert with Plo1p to regulate multiple processes such as the establishment of a bipolar mitotic spindle, transition to anaphase, placement of the actomyosin ring and proper execution of cytokinesis. We also present evidence that the effects of Pdk1p on cytokinesis are likely mediated via the fission yeast anillin-related protein, Mid1p, and the septation initiation network.
Mesh Terms:
1-Phosphatidylinositol 3-Kinase, Actomyosin, Amino Acid Sequence, Animals, Cell Division, Cell Nucleus, Cell Proliferation, Contractile Proteins, Cytokinesis, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Fungal, Genotype, Green Fluorescent Proteins, Humans, Membrane Glycoproteins, Metaphase, Mitosis, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutation, Phenotype, Phylogeny, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Time Factors
1-Phosphatidylinositol 3-Kinase, Actomyosin, Amino Acid Sequence, Animals, Cell Division, Cell Nucleus, Cell Proliferation, Contractile Proteins, Cytokinesis, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Fungal, Genotype, Green Fluorescent Proteins, Humans, Membrane Glycoproteins, Metaphase, Mitosis, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutation, Phenotype, Phylogeny, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Time Factors
Mol. Biol. Cell
Date: Jul. 01, 2005
PubMed ID: 15857958
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