Identification and characterization of a novel component of the human minichromosome maintenance complex.
Minichromosome maintenance (MCM) complex replicative helicase complexes play essential roles in DNA replication in all eukaryotes. Using a tandem affinity purification-tagging approach in human cells, we discovered a form of the MCM complex that contains a previously unstudied protein, MCM binding protein (MCM-BP). MCM-BP is conserved in multicellular eukaryotes and ... shares limited homology with MCM proteins. MCM-BP formed a complex with MCM3 to MCM7, which excluded MCM2; and, conversely, hexameric complexes of MCM2 to MCM7 lacked MCM-BP, indicating that MCM-BP can replace MCM2 in the MCM complex. MCM-BP-containing complexes exhibited increased stability under experimental conditions relative to those containing MCM2. MCM-BP also formed a complex with the MCM4/6/7 core helicase in vitro, but, unlike MCM2, did not inhibit this helicase activity. A proportion of MCM-BP bound to cellular chromatin in a cell cycle-dependent manner typical of MCM proteins, and, like other MCM subunits, preferentially associated with a cellular origin in G(1) but not in S phase. In addition, down-regulation of MCM-BP decreased the association of MCM4 with chromatin, and the chromatin association of MCM-BP was at least partially dependent on MCM4 and cdc6. The results indicate that multicellular eukaryotes contain two types of hexameric MCM complexes with unique properties and functions.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Carrier Proteins, Cell Cycle Proteins, Chromatin, DNA Helicases, DNA-Binding Proteins, Down-Regulation, G1 Phase, Gene Silencing, HeLa Cells, Humans, Immunoprecipitation, Minichromosome Maintenance Complex Component 4, Minichromosome Maintenance Complex Component 6, Minichromosome Maintenance Complex Component 7, Molecular Sequence Data, Multiprotein Complexes, Nuclear Proteins, Protein Binding, Protein Transport, Recombinant Proteins, Replication Origin, Sequence Analysis, Protein
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Carrier Proteins, Cell Cycle Proteins, Chromatin, DNA Helicases, DNA-Binding Proteins, Down-Regulation, G1 Phase, Gene Silencing, HeLa Cells, Humans, Immunoprecipitation, Minichromosome Maintenance Complex Component 4, Minichromosome Maintenance Complex Component 6, Minichromosome Maintenance Complex Component 7, Molecular Sequence Data, Multiprotein Complexes, Nuclear Proteins, Protein Binding, Protein Transport, Recombinant Proteins, Replication Origin, Sequence Analysis, Protein
Mol. Cell. Biol.
Date: Apr. 01, 2007
PubMed ID: 17296731
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