Centromeric signaling proteins boost G1 cyclin degradation and modulate cell size in budding yeast.

Cell size scales with ploidy in a great range of eukaryotes, but the underlying mechanisms remain unknown. Using various orthogonal single-cell approaches, we show that cell size increases linearly with centromere (CEN) copy number in budding yeast. This effect is due to a G1 delay mediated by increased degradation of ...
Cln3, the most upstream G1 cyclin acting at Start, and specific centromeric signaling proteins, namely Mad3 and Bub3. Mad3 binds both Cln3 and Cdc4, the adaptor component of the Skp1/Cul1/F-box (SCF) complex that targets Cln3 for degradation, these interactions being essential for the CEN-dosage dependent effects on cell size. Our results reveal a pathway that modulates cell size as a function of CEN number, and we speculate that, in cooperation with other CEN-independent mechanisms, it could assist the cell to attain efficient mass/ploidy ratios.
Mesh Terms:
Cell Cycle Proteins, Cell Division, Cell Enlargement, Cell Growth Processes, Centromere, Cyclin G1, Cyclins, G1 Phase, Gene Expression Regulation, Fungal, Membrane Glycoproteins, Nuclear Proteins, Proteolysis, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Saccharomycetales, Signal Transduction
PLoS Biol.
Date: Dec. 01, 2017
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