Rad5 Recruits Error-Prone DNA Polymerases for Mutagenic Repair of ssDNA Gaps on Undamaged Templates.
Post-replication repair (PRR) allows tolerance of chemical- and UV-induced DNA base lesions in both an error-free and an error-prone manner. In classical PRR, PCNA monoubiquitination recruits translesion synthesis (TLS) DNA polymerases that can replicate through lesions. We find that PRR responds to DNA replication stress that does not cause base ... lesions. Rad5 forms nuclear foci during normal S phase and after exposure to types of replication stress where DNA base lesions are likely absent. Rad5 binds to the sites of stressed DNA replication forks, where it recruits TLS polymerases to repair single-stranded DNA (ssDNA) gaps, preventing mitotic defects and chromosome breaks. In contrast to the prevailing view of PRR, our data indicate that Rad5 promotes both mutagenic and error-free repair of undamaged ssDNA that arises during physiological and exogenous replication stress.
Mesh Terms:
Binding Sites, Chromosomes, Fungal, DNA Breaks, Single-Stranded, DNA Helicases, DNA Repair, DNA Replication, DNA, Fungal, DNA, Single-Stranded, DNA-Directed DNA Polymerase, Mitosis, Mutation, Nucleotidyltransferases, Proliferating Cell Nuclear Antigen, Protein Binding, Recombinational DNA Repair, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitination
Binding Sites, Chromosomes, Fungal, DNA Breaks, Single-Stranded, DNA Helicases, DNA Repair, DNA Replication, DNA, Fungal, DNA, Single-Stranded, DNA-Directed DNA Polymerase, Mitosis, Mutation, Nucleotidyltransferases, Proliferating Cell Nuclear Antigen, Protein Binding, Recombinational DNA Repair, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitination
Mol. Cell
Date: Dec. 07, 2018
PubMed ID: 30733119
View in: Pubmed Google Scholar
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