A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we ...
were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
Mesh Terms:
Alleles, Cell Cycle, Cyclin B, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Genetic Complementation Test, Mutation, Nuclear Proteins, Plasmids, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Temperature, Transglutaminases
Genetics
Date: Jan. 01, 2005
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