Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.
Cdc45 is a conserved protein required for firing of replication origins and processive DNA replication. We used an in situ chromatin-binding assay to determine factors required for fission yeast Cdc45p chromatin binding. Assembly of the pre-replicative complex is essential for Cdc45p chromatin binding, but pre-replicative complex assembly occurs independently of ... Cdc45p. Fission yeast Cdc45p associates with MCM proteins in asynchronously growing cells and cells arrested in S phase by hydroxyurea, but not in cells arrested at the G2/M transition. Both hsk1+ (the fission yeast CDC7 homologue) and rad4+/ cut5+ (the fission yeast DPB11 homologue) are required for Cdc45p chromatin binding. Cdc45p also remains chromatin-bound in mutants that fail to recover from replication arrest. In summary, Cdc45p chromatin binding requires an intact pre-replicative complex as well as signaling from both the Dbf4-dependent kinase and cyclin-dependent kinases.
Mesh Terms:
Base Sequence, Cell Cycle Proteins, Chromatin, DNA Replication, DNA-Binding Proteins, MCM1 Protein, Molecular Sequence Data, Mutation, Nuclear Proteins, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Transglutaminases
Base Sequence, Cell Cycle Proteins, Chromatin, DNA Replication, DNA-Binding Proteins, MCM1 Protein, Molecular Sequence Data, Mutation, Nuclear Proteins, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Transglutaminases
Chromosoma
Date: Sep. 01, 2004
PubMed ID: 15338237
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