The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation.
A balance in the activities of the Ipl Aurora kinase and the Glc7 phosphatase is essential for normal chromosome segregation in yeast. We report here that this balance is modulated by the Set1 methyltransferase. Deletion of SET1 suppresses chromosome loss in ipl1-2 cells. Conversely, combination of SET1 and GLC7 mutations ... is lethal. Strikingly, these effects are independent of previously defined functions for Set1 in transcription initiation and histone H3 methylation. We find that Set1 is required for methylation of conserved lysines in a kinetochore protein, Dam1. Biochemical and genetic experiments indicate that Dam1 methylation inhibits Ipl1-mediated phosphorylation of flanking serines. Our studies demonstrate that Set1 has important, unexpected functions in mitosis. Moreover, our findings suggest that antagonism between lysine methylation and serine phosphorylation is a fundamental mechanism for controlling protein function.
Mesh Terms:
Amino Acid Sequence, Cell Cycle Proteins, Chromosome Deletion, Chromosome Segregation, DNA-Binding Proteins, Gene Deletion, Histone-Lysine N-Methyltransferase, Intracellular Signaling Peptides and Proteins, Methylation, Methyltransferases, Microtubule-Associated Proteins, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Protein Kinases, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors
Amino Acid Sequence, Cell Cycle Proteins, Chromosome Deletion, Chromosome Segregation, DNA-Binding Proteins, Gene Deletion, Histone-Lysine N-Methyltransferase, Intracellular Signaling Peptides and Proteins, Methylation, Methyltransferases, Microtubule-Associated Proteins, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Protein Kinases, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors
Cell
Date: Sep. 09, 2005
PubMed ID: 16143104
View in: Pubmed Google Scholar
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