NGL-2 Is a New Partner of PAR Complex in Axon Differentiation.
Neuronal polarization is pivotal for neural network formation during brain development. Axon differentiation is a hallmark of initial neuronal polarization. Here, we report that the leucine-rich repeat-containing protein netrin-G ligand-2 (NGL-2) as a polarity regulator that localizes asymmetrically in rat hippocampal neurons and is required for differentiation of the future ... axon. NGL-2 was associated with PAR complex, and this interaction resulted in local stabilization of axonal microtubules. Further study showed that the C terminal of NGL-2 binds to the PDZ domain of PAR6, and NGL-2 interacts with PAR3 and atypical PKC? (aPKC?), with PAR6 acting as a bridge or modifier. Then, NGL-2 regulates the local stabilization of microtubules and promotes axon differentiation by the aPKC?/microtubule affinity-regulating kinase 2 pathway. These findings reveal the critical role of NGL-2 in regulating axon differentiation in rat hippocampal neurons and reveal a novel partner of the PAR complex.
Mesh Terms:
Animals, Axons, Carrier Proteins, Cell Differentiation, Cells, Cultured, Female, GPI-Linked Proteins, HEK293 Cells, Hippocampus, Humans, Male, Nerve Tissue Proteins, Netrins, Rats
Animals, Axons, Carrier Proteins, Cell Differentiation, Cells, Cultured, Female, GPI-Linked Proteins, HEK293 Cells, Hippocampus, Humans, Male, Nerve Tissue Proteins, Netrins, Rats
J. Neurosci.
Date: May. 06, 2015
PubMed ID: 25948265
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