Autophagic activity dictates the cellular response to oncogenic RAS.
RAS is frequently mutated in human cancers and has opposing effects on autophagy and tumorigenesis. Identifying determinants of the cellular responses to RAS is therefore vital in cancer research. Here, we show that autophagic activity dictates the cellular response to oncogenic RAS. N-terminal Apoptosis-stimulating of p53 protein 2 (ASPP2) mediates ... RAS-induced senescence and inhibits autophagy. Oncogenic RAS-expressing ASPP2((?3/?3)) mouse embryonic fibroblasts that escape senescence express a high level of ATG5/ATG12. Consistent with the notion that autophagy levels control the cellular response to oncogenic RAS, overexpressing ATG5, but not autophagy-deficient ATG5 mutant K130R, bypasses RAS-induced senescence, whereas ATG5 or ATG3 deficiency predisposes to it. Mechanistically, ASPP2 inhibits RAS-induced autophagy by competing with ATG16 to bind ATG5/ATG12 and preventing ATG16/ATG5/ATG12 formation. Hence, ASPP2 modulates oncogenic RAS-induced autophagic activity to dictate the cellular response to RAS: to proliferate or senesce.
Mesh Terms:
Animals, Autophagy, Autophagy-Related Protein 12, Autophagy-Related Protein 5, Cellular Senescence, Embryo, Nonmammalian, Fibroblasts, Humans, Mice, Microtubule-Associated Proteins, Multiprotein Complexes, Oncogenes, Protein Binding, Protein Stability, Proteins, Proto-Oncogene Proteins p21(ras), Tumor Suppressor Proteins
Animals, Autophagy, Autophagy-Related Protein 12, Autophagy-Related Protein 5, Cellular Senescence, Embryo, Nonmammalian, Fibroblasts, Humans, Mice, Microtubule-Associated Proteins, Multiprotein Complexes, Oncogenes, Protein Binding, Protein Stability, Proteins, Proto-Oncogene Proteins p21(ras), Tumor Suppressor Proteins
Proc. Natl. Acad. Sci. U.S.A.
Date: Aug. 14, 2012
PubMed ID: 22847423
View in: Pubmed Google Scholar
Download Curated Data For This Publication
220607
Switch View:
- Interactions 2