APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle Trafficking and Neuronal Development.

Synaptic transmission is critically dependent on synaptic vesicle (SV) recycling. Although the precise mechanisms of SV retrieval are still debated, it is widely accepted that a fundamental role is played by clathrin-mediated endocytosis, a form of endocytosis that capitalizes on the clathrin/adaptor protein complex 2 (AP2) coat and several accessory ...
factors. Here, we show that the previously uncharacterized protein KIAA1107, predicted by bioinformatics analysis to be involved in the SV cycle, is an AP2-interacting clathrin-endocytosis protein (APache). We found that APache is highly enriched in the CNS and is associated with clathrin-coated vesicles via interaction with AP2. APache-silenced neurons exhibit a severe impairment of maturation at early developmental stages, reduced SV density, enlarged endosome-like structures, and defects in synaptic transmission, consistent with an impaired clathrin/AP2-mediated SV recycling. Our data implicate APache as an actor in the complex regulation of SV trafficking, neuronal development, and synaptic plasticity.
Mesh Terms:
Adaptor Protein Complex 2, Animals, Cells, Cultured, Clathrin-Coated Vesicles, Endocytosis, Female, Male, Mice, Mice, Inbred C57BL, Neurogenesis, Neurons, Protein Binding, Rats, Rats, Sprague-Dawley, Synaptic Vesicles
Cell Rep
Date: Dec. 19, 2017
Download Curated Data For This Publication
221400
Switch View:
  • Interactions 15