Enhancer Activity Requires CBP/P300 Bromodomain-Dependent Histone H3K27 Acetylation.

Acetylation of histone H3 at lysine 27 is a well-defined marker of enhancer activity. However, the functional impact of this modification at enhancers is poorly understood. Here, we use a chemical genetics approach to acutely block the function of the cAMP response element binding protein (CREB) binding protein (CBP)/P300 bromodomain in ...
models of hematological malignancies and describe a consequent loss of H3K27Ac specifically from enhancers, despite the continued presence of CBP/P300 at chromatin. Using this approach to dissect the role of H3K27Ac at enhancers, we identify a critical role for this modification in the production of enhancer RNAs and transcription of enhancer-regulated gene networks.
Mesh Terms:
Acetylation, Binding Sites, Cell Line, Tumor, Chromatin, Enhancer Elements, Genetic, Hematologic Neoplasms, Histones, Humans, Lysine, Protein Binding, Protein Domains, Protein Processing, Post-Translational, RNA, Neoplasm, Transcription, Genetic, p300-CBP Transcription Factors
Cell Rep
Date: Dec. 14, 2017
Download Curated Data For This Publication
Switch View:
  • Interactions 182