Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex.

Human Bex2 (brain expressed X-linked, hBex2) is highly expressed in the embryonic brain, but its function remains unknown. We have identified that LMO2, a LIM-domain containing transcriptional factor, specifically interacts with hBex2 but not with mouse Bex1 and Bex2. The interaction was confirmed both by pull-down with GST-hBex2 and by ...
coimmunoprecipitation assays in vivo. Using electrophoretic mobility shift assay, we have demonstrated the physical interaction of hBex2 and LMO2 as part of a DNA-binding protein complex. We have also shown that hBex2 can enhance the transcriptional activity of LMO2 in vivo. Furthermore, using mammalian two-hybrid analysis, we have identified a neuronal bHLH protein, NSCL2, as a novel binding partner for LMO2. We then showed that LMO2 could up-regulate NSCL2-dependent transcriptional activity, and hBex2 augmented this effect. Thus, hBex2 may act as a specific regulator during embryonic development by modulating the transcriptional activity of a novel E-box sequence-binding complex that contains hBex2, LMO2, NSCL2 and LDB1.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Basic Helix-Loop-Helix Transcription Factors, Cell Line, Cell Nucleus, DNA-Binding Proteins, Gene Expression Regulation, Developmental, Humans, LIM Domain Proteins, Metalloproteins, Molecular Sequence Data, Nerve Tissue Proteins, Proto-Oncogene Proteins, Sequence Alignment, Transcription Factors, Transcriptional Activation, Two-Hybrid System Techniques
Nucleic Acids Res.
Date: Nov. 30, 2005
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