Identification of proteins interacting with the rat somatostatin receptor subtype 2.
Using the yeast two hybrid system we have identified a novel protein termed somatostatin receptor interacting protein (SSTRIP) from human brain which interacts with the rat somatostatin receptor subtype 2. Interaction with the receptor C-terminus is mediated by a PSD-95/discs large/ZO-1 (PDZ) domain which exhibits high similarity to the PDZ ... domain of cortactin binding protein 1 (CortBP1). SSTRIP and CortBP1 define a novel family of multidomain proteins containing ankyrin repeats, SH3- and SH3 binding regions and a sterile alpha motif (SAM domain) in addition to the PDZ domain. Both SSTRIP and CortBP1 can be co-immunoprecipitated with the somatostatin receptor when co-expressed in HEK cells. Interestingly, co-localization of SSTR2 and CortBP1 at the plasma membrane is increased when SSTR2 is stimulated by agonists.
Mesh Terms:
Animals, Ankyrins, Binding Sites, Brain Chemistry, Carrier Proteins, Cell Line, Gene Expression, Humans, Kidney, Membrane Proteins, Nerve Tissue Proteins, Phosphoproteins, Protein Structure, Tertiary, Rats, Receptors, Somatostatin, Two-Hybrid System Techniques, Zonula Occludens-1 Protein, src Homology Domains
Animals, Ankyrins, Binding Sites, Brain Chemistry, Carrier Proteins, Cell Line, Gene Expression, Humans, Kidney, Membrane Proteins, Nerve Tissue Proteins, Phosphoproteins, Protein Structure, Tertiary, Rats, Receptors, Somatostatin, Two-Hybrid System Techniques, Zonula Occludens-1 Protein, src Homology Domains
J. Physiol. Paris
Date: Nov. 23, 2000
PubMed ID: 11087996
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