Identification of COMMD1 as a novel lamin A binding partner.
Lamin A, which is encoded by the LMNA gene, regulates gene expression and genome stability through interactions with a variety of proteins. Mutations in LMNA lead to a diverse set of inherited human diseases, collectively referred to as laminopathies. To gain insight into the protein interactions of lamin A, a ... yeast two?hybrid screen was conducted using the carboxy?terminus of lamin A. The screen identified copper metabolism MURR1 domain?containing 1 (COMMD1) as a novel lamin A binding partner. Colocalization experiments using fluorescent confocal microscopy revealed that COMMD1 colocalized with lamin A in 293 cells. Furthermore, the COMMD1?lamin A protein interaction was also demonstrated in co?immunoprecipitation experiments. Collectively, the present study demonstrated a physical interaction between COMMD1 and lamin A, which may aid to elucidate the mechanisms of lamin A in the aging process.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Cell Line, Humans, Lamin Type A, Protein Binding, Protein Interaction Maps, Two-Hybrid System Techniques
Adaptor Proteins, Signal Transducing, Cell Line, Humans, Lamin Type A, Protein Binding, Protein Interaction Maps, Two-Hybrid System Techniques
Mol Med Rep
Date: Aug. 01, 2019
PubMed ID: 31257505
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